[blaVIM-2 gene detection in metallo-beta-lactamase-producing Pseudomonas aeruginosa strains isolated in an intensive care unit in Ciudad Bolívar, Venezuela]

Guevara, Armando · de Waard, Jacobus · Araque, María

2009-09-23

2009-08

Abstract · Research Article

spa

Pseudomonas aeruginosa · Enzymology · beta-Lactamases · Genetics · Strains · Pseudomonas · Intensive Care Units · Genes · Venezuela · Intensive Care

Adolescent · Adult · Anti-Bacterial Agents / pharmacology · Child · Child, Preschool · Cross Infection / microbiology · Disk Diffusion Antimicrobial Tests · Drug Resistance, Bacterial / genetics · Female · Humans · Intensive Care Units · Male · Middle Aged · Phenotype · Polymerase Chain Reaction · Pseudomonas Infections / microbiology · Pseudomonas aeruginosa / drug effects · Pseudomonas aeruginosa / enzymology · Venezuela · Young Adult · beta-Lactamases / biosynthesis · beta-Lactamases / genetics

Pseudomonas aeruginosa / enzymology · beta-Lactamases / genetics

19802400

Ten Pseudomonas aeruginosa strains with resistance to broad-spectrum cephalosporin and carbapenems were studied to determine the presence of genes that mediate the production of metallo-beta-lactamases. These strains were isolated from patients with nosocomial infection at the Intensive Care Unit of the Complejo Hospitalario "Ruiz y Paéz" of Ciudad Bolívar, Bolívar State, Venezuela, from 2003 to 2006. In all isolates a metallo-enzyme activity was detected by using the double disk synergism test. PCR amplification of genes encoding the families IMP, VIM and SPM metallo-beta-lactamases showed the presence of a blaVIM gene in all strains studied. DNA sequencing revealed that all isolates showed the presence of blaVIM-2. These results suggest that it is necessary to keep these strains under epidemiologic surveillance, establish laboratory strategies for opportune detection and the implementation of new policies to ensure the appropriate use of antibiotics in this institution.

Revista chilena de infectología : órgano oficial de la Sociedad Chilena de Infectología

0716-1018

NLM

MEDLINE

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